段硕

发布者:生命科学学院发布时间:2022-06-21浏览次数:

个人信息:

段硕,男,副教授,江西省杰青基金获得者,硕士研究生导师(招生方向:园艺学、生物医学工程)。以柑橘细菌性病害(柑橘溃疡病、柑橘黄龙病)为研究对象进行致病机理、病原检测、抗耐育种与防治管理等方向的研究。主要涉及病原致病机制与宿主互作、抑菌物质筛选与抗耐分子遗传育种、柑橘根叶际微生物生防菌挖掘与应用、柑橘砧木介导系统抗病性等研究领域。

如果对以上研究领域感兴趣,欢迎报考本人团队共同研究探索!

联系邮箱: shuoduan@gnnu.edu.cn

教育经历:

2013.09 - 2018.07    博士,植物病理学,西南大学2014.08-2017.07 佛罗里达大学(CSC-联合培养项目)

2010.09 - 2013.07  硕士,植物病理学,西南大学

2006.09 - 2010.07 学士,植物保护,西南大学

工作经历:

2020.01-至今,赣南师范大学生命科学学院

2017.08- 2019.09,美国佛罗里达大学柑橘研究与教育中心,博士后

教授课程:

本科生课程:《植物病理学》《植物保护通论》《农业气象学》《土壤肥料学》

研究生课程:《园艺植物逆境生物学》《高级分子生物学技术研究进展》

主持及主要参与项目:

1) 江西省自然基金杰出青年基金项目,维管系统介导柑橘遗传转化根系提升植株抗病性的研究,主持;

2) 国家青年科学基金项目, CsPDT 基因调控溃疡病表型形成机制的研究,主持;

3) 横向课题,寡糖类生物农药的杀溃疡病评价,主持;

4) 美国农业部研究计划,Consequences of genome editing the susceptibility gene CsLOB1 for citrus bacterial canker,参与

发表论文 (*通讯作者,#共同第一)

1) Zhang, SY., Luo, RF., Wu, YX., Zhang, TT., Yusuf, A., Wang, N., Li, M., Duan, S*. (2025). Establishment and application of high-pressure propagation breeding (HPPB)-mediated genetic transformation system in citrus rootstocks. Plant Biotechnology JournalAccepted

2) Yusuf, A., Li, M., Zhang, SY., Ajibade, F.O., Luo, RF., Wu, YX., Zhang, TT., Ugya, AY., Zhang, Y., Duan, S*. (2025). Harnessing plant–microbe interactions: Strategies for enhancing resilience and nutrient acquisition for sustainable agriculture. Frontiers in Plant ScienceAccepted

3) Duan, S., Long, Y., Cheng, S., Li, J., Ouyang, Z., & Wang, N. (2022). Rapid Evaluation of the Resistance of Citrus Germplasms Against Xanthomonas citri subsp. citri. Phytopathology, 112(4), 765–774.

4) Guo, B., Duan, S#., Liu, F., & Fu, Z. Q. (2022). A vigilant gliding bird protects plants. Trends in biochemical sciences, 47(10), 819–821.

5) Ma, H., Meng, X., Xu, K., Li, M., Gmitter, F. G., Jr, Liu, N., Gai, Y., Huang, S., Wang, M., Wang, M., Wang, N., Xu, H., Liu, J., Sun, X., Duan, S*. (2022). Highly efficient hairy root genetic transformation and applications in citrus. Frontiers in plant science, 13, 1039094.

6) Li, M., Qin, HK., Long, YF., Cheng, MQ., Li, L., Huang, AJ., Wang, N., Duan, S*. (2022). Field Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a, JoVE Journal.

7) Duan, S., Xu, Z., Li, X. Y., Liao, P., Qin, H. K., Mao, Y. P., Dai, W. S., Ma, H. J., & Bao, M. L. (2022). Dodder-transmitted mobile systemic signals activate a salt-stress response characterized by a transcriptome change in Citrus sinensis. Frontiers in plant science, 13, 986365.

8) Duan, S., Jia, H., Pang, Z., Teper, D., White, F., Jones, J., Zhou, C., & Wang, N. (2018). Functional characterization of the citrus canker susceptibility gene CsLOB1. Molecular plant pathology, 19(8), 1908–1916.

9) Long, Y., Luo, R., Xu, Z., Cheng, S., Li, L., Ma, H., Bao, M., Li, M., Ouyang, Z., Wang, N., & Duan, S*. (2022). A Fluorescent Reporter-Based Evaluation Assay for Antibacterial Components Against Xanthomonas citri subsp. citri. Frontiers in microbiology, 13, 864963.

10) Wang, Y., Duan, S#., Xu, J., Long, YF., Zhang, SY., Li, SX., Wu, LJ., Zhang, YZ. (2023). Comparison of the colonization ability of Burkholderia strain B23 in the citrus rhizoplane and rhizosphere and assessment of the underlying mechanisms using full-length 16S rDNA amplicon and metatranscriptomic analyses, Microbial Biotechnology.

11) Lin, H., Liang, Y., Kaliaperumal, K., Xiong, Q., Duan, S., Jiang, Y., Zhang, J. (2023) Linoleic acid from the endophytic fungus Diaporthe sp. HT-79 inhibits the growth of Xanthomonas citri subsp. citri by destructing the cell membrane and producing reactive oxygen species (ROS). Pesticide Biochemistry and Physiology.

12) Hu, Y., Duan, S., Zhang, Y., Shantharaj, D., Jones, J. B., & Wang, N. (2016). Temporal Transcription Profiling of Sweet Orange in Response to PthA4-Mediated Xanthomonas citri subsp. citri Infection. Phytopathology, 106(5), 442–451.

13) Li, J., Pang, Z., Duan, S., Lee, D., Kolbasov, V. G., & Wang, N. (2019). The in Planta Effective Concentration of Oxytetracycline Against ' Candidatus Liberibacter asiaticus' for Suppression of Citrus Huanglongbing. Phytopathology, 109(12), 2046–2054.

14) Gill RA., Li XL., Duan S., Xing Q., Xing RM. (2024) Citrus threat huanglongbing (HLB) - Could the rootstock provide the cure? Frontiers in plant science., doi.org/10.3389/fpls.2024.1330846

15) Wang, XF., Chen, JY., Tan, J., Duan, S., Deng, XL., Chen, JC. and Zhou, CY. (2015) High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘ Candidatus Liberibacter asiaticus., Journal of Integrative Agriculture., 14, 2002-2010.

16) Hu W., Zhao CF., Zheng RK., Duan S., Lu ZJ., Zhang ZX., Yi L., Zhang N. (2023) Serratia marcescens induces apoptosis in Diaphorina citri gut cells via reactive oxygen speciesmediated oxidative stress, Pest management Science., doi: 10.1002/ps.7787

17) Peng, T., Yuan, YZ., Huang, AJ., He, J., Fu, SM., Duan, S., Yi, L., Yuan, CY., Yuan, HZ., Wang, XF., Zhou, CY. (2023) Interaction between flagellum of Candidatus Liberibacter asiaticus (CLas) and vitellogenin-like protein of Diaphorina citri significantly influences CLas titer, Frontier in Microbiology.

18) Zhang. J., Zhang, JY, Lin, HT., Liang, Y., Kaliaperumal, K., Xiong, Q., Duan, S., Jiang, YM. (2023) Semiliquidambar chingii is a highly potent antibacterial plant resource against Xanthomonas citri subsp. citri: Insights into the possible mechanisms of action, chemical basis, and synergistic effect of bioactive compounds, Industrial Crops and Products.

19) Meng, S., Huang, S., Liu, J., Gai, Y., Li, M., Duan, S., Zhang, S., Sun, X., Yang, Q., Wang, Y., Xu, K., & Ma, H. (2022). Histone Methylation Is Required for Virulence, Conidiation, and Multi-Stress Resistance of Alternaria alternata. Frontiers in microbiology, 13, 924476.

20) Huang, S., Jia, Z., Li, H., Zhang, S., Shen, J., Gai, Y., Jiao, C., Sun, X., Duan, S., Wang, M., Ma, H. (2022) ACT-Toxin, the Key Effector for the Virulence of Alternaria alternata Tangerine Pathotype to Specific Citrus Species. Agronomy. 12(12):3181.

21) 段硕, 李敏, 陶珍珍, 宋震, 李中安, 周常勇. (2013) 柑橘碎叶病毒外壳蛋白基因的原核表达, 果树学报, 30 (5):744-747

22) 段硕, 周常勇. (2012)  柑桔疮痂病研究进展, 中国南方果树

23) 李敏, 段硕, 李中安, 周彦, 周常勇等. (2013) 叶片微形态结构特征与柑桔溃疡病抗性的关系, 中国南方果树, 42(2): 1–5.

研发专利

1) 一种基于RPA-CRISPR-Cas12a体系检测黄龙病菌的方法, CN202210732514.0

2) 一种芸香科植物的遗传转化体系、遗传转化方法和应用,CN114657207A

3) 一种高通量测定柑橘溃疡病菌杀菌剂活性的方法,CN114350741 B

4) 一种柑橘基因突变体及其应用,CN202210577815.0

5) 一种快速评估柑橘品种对溃疡病抗性的方法,CN202111567079.2

6) 一种柑橘的病毒接种方法,CN202210097841.3

7) 柑橘碎叶病毒的RT-LAMP检测方法,201210450791.9

会议报告

1) Duan, S. Exploring antimicrobial peptides for the control of citrus Huanglongbing disease- a strategy for genetic transformation and evaluation of disease resistance in rootstocks, 15th  International citrus congress .(2024), 济州岛,韩国

2) Duan, S. Direct evidence of CsLOB1 being the susceptibility gene of citrus canker caused by Xanthomonas citri, American Phytopathological Society Annual Meeting (2017),德克萨斯圣安东尼奥, 美国