陈玮

发布者:生命科学学院发布时间:2021-11-18浏览次数:



陈玮,讲师,硕士生导师,2020年毕业于福建农林大学农业昆虫与害虫防治专业,擅长挖掘组学数据(转录组-蛋白质组-代谢组)和构建植物/昆虫转基因材料(CRISPR/Cas9),从DNA-mRNA-Protein多层次研究专食性害虫小菜蛾十字花科植物、专食性害虫柑橘木虱与芸香科植物互作的分子机理,研究方向包括:

1. 脱落酸信号通路介导寄主植物抵御害虫的分子机理;

2. 专食性害虫适应寄主植物天然防御的分子机理;

3. 基于植物次生挥发物质对专食性害虫诱杀的田间应用。

一、学习与工作经历

202007-至今, 赣南师范大学生命科学学院,副教授

201609-202006月,福建农林大学植物保护学院应用生态研究所  博士  尤民生;

201309-201606月,福建农林大学植物保护学院应用生态研究所  硕士  何玮毅;

200909-201306月,福建农林大学植物保护学院  本科  詹家绥。

二、主持科研项目:

[1] 国家自然科学基金地区项目小菜蛾口腔分泌蛋白GSS1与脱落酸合成酶aba1互作调控植物防御反应的分子机理(32260721),2023.01-2026.1233万,主持

[2] 江西省自然科学基金青年项目,CLas病菌利用宿主泛素化调控网格蛋白介导的内吞途径进入木虱中肠细胞的分子机制(20232BAB215035),2023.07-2026.0710万,主持

[3] 闽台作物有害生物生态防控国家重点实验室开放课题基金,植保素介导十字花科植物抗虫的分子机理(SKL2022001),2021.12-2024.125万,主持

[4] 江西省教育厅,小菜蛾硫苷硫酸酯酶GSS1转录因子的鉴定及功能探究(GJJ461832),2020.01-2023.122万,主持已结题

[5] 赣南师范大学人才引进科研启动经费,昆虫分泌口腔蛋白重要基因的鉴定及其功能解析(414767),2020.07-2023.0710万,主持已结题

[6] 国家自然科学基金面上项目,柑橘黄龙病菌SDEs介导柑橘木虱中肠上皮细胞囊泡转运机制研究(322725492023.12-2026.1254万元,参与

[7] 国家自然科学基金地区项目,调控植物防御与柑橘木虱取食的唾液效应因子鉴定及功能解析(32160634),2022.01-2025.1235万,参与

三、近年主要研究成果

(一)第一作者和通讯作者

[1] Yun M., Xiong Y., Wang Z.,Xie L.,Ye H.,Yuan X.,He W.,Chen B.*, Lu Z.*, Chen W*. 2025 An insect oral secretion protein and its related core peptide induce the host plant's endogenous abscisic acid to enhance resistance against insect. Journal of Agricultural and Food Chemistry 73, 11452-11465

[2] Qiao Q., Feng H.,Jiao L., Zaheer U., ...... Chen W.*, He W.*, Yang F.*, 2024 Bacteria derived from diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), gut regurgitant negatively regulate glucose oxidase-mediated anti-defense against host plant. Insects 15, 1001.

[3] Li S., Lin G., Wen H.,… Chen W.*, He W.*, 2024 Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal gene and sex chromosomes in the diamondback moth, Plutella xylostella. Journal of Integrative Agriculture 23(9), 3089-3103.

[4] Xie L., Zeng X., Amir M., Zou X., Yuan J., Yu H., Lu Z., Chen W. 2023 Clathrin heavy chain is involved in infection of Candidatus Liberibacter asiaticus in the host vector Diaphorina citri. Insect Science DOI: 10.1111/1744-7917.13305.

[5] Yang F., Jing X., Dong R., Zhou L., Xu X., Dong Y, Zhang L, Zheng L., Lai Y., Chen Y., Lin L., Ma X., You M., Chen W.* and He W.* 2023 Glucose oxidase of a crucifer-specialized insect: a potential role in suppressing plant defense via modulating antagonistic plant hormones. Journal of Agricultural and Food Chemistry 71(45) 17469–17483.

[6] Chen W.*, Amir M.B., Liao Y., Yu H., He W.*, Lu Z. * 2023. New insights into the Plutella xylostella detoxifying enzymes: sequence evolution, structural similarity, functional diversity, and application prospects of glucosinolate sulfatases. Journal of Agricultural and Food Chemistry 71(29) 10952–10969.

[7] Xiong Y.,† Jiang C.†, Amir M.B., Dong Y., Xie L., Liao Y., He W.*, Lu Z.*, Chen W.,* 2022. Antibody-based methods reveal the protein expression properties of glucosinolate sulfatase 1 and 2 in Plutella xylostella, Journal of Insect Science 22(6): 5; 1–10

[8] Chen W., Saqib H., Xu X., Dong Y., Zheng L., Lai Y., Jing X., Lu Z., Sun L., You M.,* He W.*., 2022. Glucosinolate sulfatases−sulfatase-modifying factors system enables a crucifer-specialized moth to pre-detoxify defensive glucosinolate of the host plant. Journal of Agricultural and Food Chemistry 70(36): 11179–11191

[9] Chen W., Dong Y., Zheng L., Lai Y., Li F., Zhou L., Wang B., You M. and He W., 2022. An inducible gene from glycoside hydrolase one family of Plutella xylostella decreases larval survival when feeding on host plant. Frontiers in Physiology 13:1013092.

[10] Chen W., Dong Y., Saqib H.S.A., Vasseur L., Zhou W., Zheng L., Lai Y., Ma X., Lin L., Xu X., Bai J., He W., You M., 2020. Functions of duplicated glucosinolate sulfatases in the development and host adaptation of Plutella xylostella. Insect Biochemistry and Molecular Biology 119: 103316.

[11] Chen W., Yang F., Xu X., Kumar U., He W., You M., 2019. Genetic control of Plutella xylostella in omics era. Archives of Insect Biochemistry and Physiology 102(3): e21621.

[12] Chen W., Dong Y., Lin L., Saqib H.S.A., Ma X., Xu X., Zhang L., Jing X., Peng L., Wang Y., Vasseur L., He W., You M., 2019. Implication for DNA methylation involved in the host transfer of diamondback moth, Plutella xylostella (L.). Archives of Insect Biochemistry and Physiology 102(2): e21600.


(二)非第一作者或通讯作者

[1] Wanxin Xing, Lianyun Lin, Zijing Wang, Lei Xiong, Hadiatullah Hadiatullah, Wei Chen, Shijun You, Bernard Moussian, Yiwen Wang, Zhiguang Yuchi. 2023. Expression and purification of snustorr snarlik protein from Plutella xylostella. Protein Expression and Purification, 206:106256

[2] Zhang J.B., Zou X.J, Zhang Q., Wang A.Y., Amir M.B., Du Y.M., Liu X.Q., Chen W., Lu Z.J., Yu H.Z., 2023. Quantitative ubiquitylome crosstalk with proteome analysis revealed cytoskeleton proteins influence CLas pathogen infection in Diaphorina citri. International Journal of Biological Macromolecules, 232: 123411.

[3] Xu X., Yang J., Harvey-Samuel T., Huang Y., Asad M., Chen W., He W., Yang G., Alphey L., You M., 2020. Identification and characterization of the vasa gene and its promoter in the diamondback moth, Plutella xylostella. Insect Biochemistry and Molecular Biology 122: 103371.

[4] Hameed M.S., Jing X., Chen W., Chen J., Vasseur L., Wang-Pruski G., Yang G., 2019. Function and characterization of the argonaute2 gene in the RNA interference pathway in the diamondback moth. Archives of Biochemistry and Molecular Biology 10: 035-051.

[5] Zhang L., Jing X., Chen W., Wang Y., Lin J., Zheng L., Dong Y., Zhou L., Li F., Yang F., Peng L., Vasseur L., He W., You M., 2019. Host plant-derived miRNAs potentially modulate the development of a cosmopolitan insect pest, Plutella xylostella. Biomolecules 9: 602.

[6] Zhang L., Jing X., Chen W., Bai J., Vasseur L., He W., You M., 2019. Selection of reference genes for expression analysis of plant-derived microRNAs in Plutella xylostella using qRT-PCR and ddPCR. PLoS One 14: e0220475.

[7] Lin L., # Liu C., # Qin J., # Wang J., Dong S., Chen W., He W., Gao Q., You M., Yuchi Z., 2018. Crystal structure of ryanodine receptor N-terminal domain from Plutella xylostella reveals two potential species-specific insecticide-targeting sites. Insect Biochemistry and Molecular Biology 92: 73-83 (# joint first author).

[8] Ma X., # He W.,# Chen W., Xu X., Qi W., Zou M., You Y., Baxter S W., Wang P., You M., 2017. Structure and expression of sulfatase and sulfatase modifying factor genes in the diamondback moth, Plutella xylostella (L.). Insect Science 25: 946-958 (# joint first author).

[9] Qi W.,# Ma X.,# He W.,# Chen W., Zou M., Gurr G.M., Vasseur L., You M., 2016. Characterization and expression profiling of ATP-binding cassette transporter genes in the diamondback moth, Plutella xylostella (L.). BMC Genomics 17: 760 (# joint first author).


四、国家发明专利和新品种权:

1. 发明人:Wei CHENYuan LIAOYu XIONGQian GUOMengjie WUHongjing ZHONGXin RENRuijun XUXin ZHONGZhanjun LU. 发明专利名称:INSECT-DERIVED SHORT PEPTIDE FOR IMPROVING ABSCISIC ACID (ABA) CONTENT IN CRUCIFEROUS PLANT,状态:受理20240517日,授权国别:美国;受理专利号:23399

2. 发明人:陈玮,王琢冰,谢廉颉,苏欣彤,刘宇,刘小强,刘映雪,余海中,卢占军;发明专利名称:DcPICALM 基因在阻断柑橘黄龙病传播中的应用,状态:受理20240612日;授权国别:中国;受理专利号:202410757785.0

3. 发明人:廖远、陈玮、熊余、郭骞、吴梦洁、钟鸿婧、任鑫、许瑞俊、钟欣、卢占军;发明专利名称:一种激发十字花科植物脱落酸含量的昆虫源短肽,状态:受理20230313日;授权国别:中国;受理专利号:202310232365.6

4. 发明人:董玉红,陈玮,熊余,谢廉颉,钟欣,周洋飞,卢占军,何玮毅,朱博;发明专利名称:GSS1蛋白质作为小菜蛾口腔分泌蛋白的内参蛋白的应用,状态:受理2022.05.10;授权国别:中国;专利号:202210505729.9

5. 发明人:陈玮,郑玲,董玉红,熊余,赖颖芳,何玮毅,尤民生,卢占军;发明专利名称:一种小菜蛾蛋白Western Blot定量检测方法;状态:授权2021.05.24;授权国别:中国;专利号:202011577797.3

6. 昆虫-植物互作生物学观测简易装置(排名第1),专利申请号:2019209446800(已授权).

五、研制标准:

六、出版专著:

七、获奖:

1. 中国国际大学生创新大赛(2024) 本科生创意组-国赛金奖,2024.10,第一指导老师

2. 江西省园艺学会2023年度优秀会员,2023.12

3. 全国第九届“互联网+”大学生创新创业大赛-铜奖,2023.10,第一指导老师

4. 赣南师范大学第九届“互联网+”大学生创新创业大赛,2023.10,优秀指导教师

5. 第八届“创在江西”青年创新创业大赛银奖(总排名第三)2023.11,第一指导老师

6. 第八届全国大学生生命科学竞赛二等奖,2023.08,第一指导老师

7. 首届江西省科学实验展演汇演活动 一等奖,2023.07,第一指导老师

8. 江西省第九届“互联网+”大学生创新创业大赛-金奖,2023.07,第一指导老师

9. 赣南师范大学第八届“互联网+”大学生创新创业大赛2022.10,优秀指导教师


八、学术会议:

1. 15届国际柑橘年会(2024,每4年一届),报告题目:Molecular Mechanism of DcChc and DcPICALM Facilitating CLas Infection in the Midgut Cell of Diaphorina citri主办方:国际柑橘学会(International Citrus CongressISC)和韩国柑橘和亚热带水果学会(Korean Citrus and Subtropical Fruit Society),会议时间:2024.11.10-11.15

2. 科学预防虫灾,生态控制损失-华中昆虫学术研讨会作分会报告,报告题目:GSS-Sumf体系调控小菜蛾解毒寄主植物硫苷的分子机理,主办方:江西省昆虫学会,会议时间:2022.11.05

3. 第四届害虫生态防控青年学术论坛作分会报告,报告题目:福祸相依—昆虫解毒酶降解的蛋白肽诱发植物激素介导的防御,主办单位:中国昆虫学会农业昆虫专业委员会,会议时间:2023.06.02

4. 2023华中昆虫学术交流暨全国害虫绿色防控研讨会作会议报告,报告题目:福祸相依—昆虫解毒酶降解的蛋白肽诱发植物激素介导的防御,主办单位:河南省科学技术协会,会议时间:2023.07.10